Immunoelectron microscopy reveals the presence of neurofilament proteins in retinal terminals undergoing dark degeneration.

ABSTRACT

After nerve crushing or section, the distal stump undergoes morphological changes described as Wallerian degeneration (WD). Immediately after nerve injury, early ultrastructural alterations occur in the terminal boutons, a process known as terminal degeneration (TD), which occurs before degeneration of the axon and leads to electrophysiological impairment. In this study we investigated the presence of neurofilament (NF) proteins in TD and compared the results with degeneration in the optic nerve. Young adult Wistar rats were submitted to bilateral enucleation and perfused after 24 h, 48 h and 1 week. Optic nerves (ON) and superior colliculus (SC) segments were processed for electron microscopy (EM) and immunoelectron microscopy (IEM) for NF subunits. Analysis of ultrathin sections of SC, at 24 h, revealed terminals undergoing TD. At 48 h and 1 week after enucleation, there was a clear increase in the number of degenerating terminals. The cytoarchitecture of the optic nerve did not change considerably at 24 h, but it was progressively altered at 48 h and 1 week after enucleation, when we observed intense astrogliosis, and most fibers exhibited dark degeneration (DD). The IEM for the NF subunits of normal ON showed gold particles located along the filaments, but we did not observe labeling for neurofilament proteins in normal retinal terminals. However, 48 h after lesion, we observed immunogold particles for the NF proteins in fibers undergoing DD and on terminals undergoing TD. Therefore, we can conclude that NF proteins participate in the process of TD, and this event occurs before complete axonal degeneration, suggesting different mechanisms for TD and DD.