Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats.
Ulus Travma Acil Cerrahi Derg
; 15(1): 1-6, 2009 Jan.
Article
en En
| MEDLINE
| ID: mdl-19130331
ABSTRACT
BACKGROUND:
The second hit in trauma leads to an exaggerated inflammatory response and multiple organ failure. Infection following burn injury is a useful model for two-hit trauma studies. The aim of this study was to investigate the effect of N-acetylcysteine (NAC) treatment as an antioxidant in a two-hit trauma model.METHODS:
30% scalding burn injury was performed in 45 rats and cecal ligation-puncture (CLP) was performed 72 hours later. Groups were allocated as follows Group I No treatment was performed; Group II Rats were treated with 150 mg/kg/day i.p. NAC for 72 h following CLP; Group III Rats were treated with 150 mg/kg/day i.p. NAC for 6 days following thermal injury. Tissue samples were collected to study the tissue malonyldialdehyde (MDA) and glutathione (GSH) levels, and for histopathological examination on day 7.RESULTS:
No difference in mortality between groups was detected. Tissue MDA levels significantly decreased in the liver (p=0.01, p=0.02) and ileum (p=0.01, p=0.02) in the treatment groups. Lung tissue GSH levels were found to be significantly increased in Group II (p=0.02). Lung injury scores were decreased in Group II (p=0.005) compared to the control group.CONCLUSION:
NAC attenuated tissue oxidative stress level and remote organ injury in two-hit trauma. Further experimental and clinical studies on this subject are necessary.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Acetilcisteína
/
Estrés Oxidativo
/
Glutatión
/
Malondialdehído
/
Antioxidantes
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Ulus Travma Acil Cerrahi Derg
Año:
2009
Tipo del documento:
Article
País de afiliación:
Turquía