Morphology of the bone marrow, spleen and liver during hematopoietic cell mobilization with cyclophosphamide in mice.
Folia Histochem Cytobiol
; 46(4): 501-9, 2008.
Article
en En
| MEDLINE
| ID: mdl-19141405
ABSTRACT
Cyclophosphamide (CY), the agent with cytoreductive activity, is widely exploited in cancer chemotherapy, and can be used alone or in combination with various cytokines and growth factors to stimulate the egress of hematopoietic stem/progenitor cells (HSPC) from the BM compartment. The aim of the present study was to exam the morphology and ultrastructure of the bone marrow, spleen and liver of mice injected intraperitoneally with a single dose of cyclophosphamide (200 mg/kg bw) and the localization of cells expressing markers of early hematopoietic cells in studied organs and the peripheral blood. We observed that the CY-induced morphological changes in the BM and spleen were reconstructed on day 4. of experiment, and the spleen was repopulated by HSPC on the 6th day. In this time, the highest number of c-Kit-R-positive cells was determined by flow cytometry in the peripheral blood. The results confirmed, that the egress of HSPC from the bone marrow into the peripheral blood was delayed compared to mice treated with G-CSF or GCS-F plus CY.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Médula Ósea
/
Movilización de Célula Madre Hematopoyética
/
Ciclofosfamida
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
Folia Histochem Cytobiol
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Polonia