Heme oxygenase-1 increases endothelial progenitor cells.
Arterioscler Thromb Vasc Biol
; 29(10): 1537-42, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19542024
ABSTRACT
OBJECTIVE:
Induction of heme oxygenase-1 (HO-1) protects against atherosclerotic disease in part by promoting reendothelialization. As endothelial progenitor cells (EPCs) contribute to reendothelialization, we examined the role of HO-1 on bone marrow and circulating EPCs. METHODS ANDRESULTS:
In a rabbit model of aortic balloon injury, pharmacological induction of HO-1 enhanced reendothelialization at sites with and without adjacent blood vessels, the latter indicative of a contribution by EPCs. Coinciding with maximal HO-1 induction in the injured vessel, plasma concentrations of bilirubin and the numbers of circulating progenitor cells were elevated. Both processes were abolished by cotreatment of the animals with an inhibitor of HO-1. Inducers of HO-1 promoted bone marrow cells to form progenitor cell colonies, and Flk1(+)/Sca-1(+)-cells to adhere to the luminal surface of the injured vessel. In noninjured mice, HO-1 inducers also increased bone marrow and circulating EPCs, and the ability of these cells to differentiate and form colonies. Compared to wild-type mice, bone marrow cells from HO-1(-/-) mice generated fewer endothelial colony-forming cells, and HO-1 inducers failed to promote CFU-Hill colony formation.CONCLUSIONS:
These findings suggest that HO-1 contributes to vascular repair by increasing circulating EPCs derived from the bone marrow.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
/
Células Endoteliales
/
Hemo-Oxigenasa 1
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Arterioscler Thromb Vasc Biol
Asunto de la revista:
ANGIOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Australia