Acute effects of testosterone infusion on the serum luteinizing hormone profile in eumenorrheic and polycystic ovary syndrome adolescents.
J Clin Endocrinol Metab
; 94(9): 3602-10, 2009 Sep.
Article
en En
| MEDLINE
| ID: mdl-19567528
ABSTRACT
CONTEXT Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females. OBJECTIVE:
Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents. DESIGN ANDSETTING:
We conducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center.PARTICIPANTS:
Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months. INTERVENTION Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight. MAIN OUTCOMEMEASURES:
LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses.RESULTS:
On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P < 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion.CONCLUSIONS:
PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Síndrome del Ovario Poliquístico
/
Testosterona
/
Hormona Luteinizante
Tipo de estudio:
Clinical_trials
/
Observational_studies
Límite:
Adolescent
/
Female
/
Humans
Idioma:
En
Revista:
J Clin Endocrinol Metab
Año:
2009
Tipo del documento:
Article
País de afiliación:
Argentina