Double transgenic mice crossed GFP-LC3 transgenic mice with alphaMyHC-mCherry-LC3 transgenic mice are a new and useful tool to examine the role of autophagy in the heart.
Circ J
; 74(1): 203-6, 2010 Jan.
Article
en En
| MEDLINE
| ID: mdl-19966504
ABSTRACT
BACKGROUND:
The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. METHODS ANDRESULTS:
We generated transgenic mice expressing mCherry-LC3 under alphaMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation.CONCLUSIONS:
This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74 203 - 206).
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Autofagia
/
Cadenas Pesadas de Miosina
/
Proteínas Fluorescentes Verdes
/
Modelos Animales de Enfermedad
/
Proteínas Asociadas a Microtúbulos
/
Miocardio
Límite:
Animals
Idioma:
En
Revista:
Circ J
Asunto de la revista:
ANGIOLOGIA
/
CARDIOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón