Genomic loss of imprinting in first-trimester human placenta.
Am J Obstet Gynecol
; 202(4): 391.e1-8, 2010 Apr.
Article
en En
| MEDLINE
| ID: mdl-20350649
OBJECTIVE: The purpose of this study was to investigate imprinting patterns in first-trimester human placentas. STUDY DESIGN: Using samples of 17 first-trimester and 14 term placentas from uncomplicated pregnancies, we assessed loss of imprinting (LOI) at the RNA level in a panel of 14 genes that are known to be imprinted in the placenta with the use of a quantitative allele-specific reverse transcriptase polymerase chain reaction analysis of those genes that contained readout single nucleotide polymorphisms in their transcripts. RESULTS: There is significant LOI (ie, biallelic expression) in all 14 genes in first-trimester placentas. LOI was more variable and generally at lower levels at term. Although there is little difference in gene expression, the level of LOI is higher in the first-trimester placentas, compared with term placentas. CONCLUSION: Genomic imprinting appears to be a dynamic maturational process across gestation in human placenta. In contrast with prevailing theories, epigenetic imprints may continue to evolve past 12 weeks of gestation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Placenta
/
Primer Trimestre del Embarazo
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Impresión Genómica
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Genómica
Límite:
Female
/
Humans
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Pregnancy
Idioma:
En
Revista:
Am J Obstet Gynecol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos