Taxol content comparison in different parts of Taxus madia and Taxus chinensis var. mairei by HPLC.

ABSTRACT

OBJECTIVE: To analysis and compare the taxol content in different parts of Taxus madia and Taxus chinensis var. mairei by High Performance Liquid Chromatography (HPLC). METHODS: 85% EtOH and CH2Cl2 were used for the extraction of taxol. By HPLC, the methodology study and taxol content investigation were performed. RESULTS: The taxol was extracted successfully. One simple and reliable methodology was built up. Basing on these, the taxol content in these two Taxus spp. were analysed and compared, among of which the leaf of Taxus chinensis var. mairei has the highest taxol content (5.18 x 10(-5), w/w). CONCLUSION: Taxol and its content in the original plants can be simply and reliably extracted and investigated by these methods, which also can provided the scientific basis for the rational development of Taxus spp.