[Dynamic observation on splenocyte subsets in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus].

ABSTRACT

OBJECTIVE: To dynamically observe changes of subsets of splenocytes in mice immunized with recombinant Bifidobacteria bifidum (Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus (Eg). METHODS: BALB/c mice were vaccinated by 5 x 10(8) colony forming unit (CFU) orally and 5 x 10(5) CFU intranasally respectively. Mice were killed on week 0, 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 after immunization respectively, and spleens were separated for cell preparation. CD4+ and CD8+ T cells were determined by flow cytometry (FCM), with MRS as control. RESULTS: In the oral immunization group, CD4+ cells showed a significant increase during the 4th-10th week after vaccination, and reached the highest level on the 6th week, whereas no obvious changes in CD8+ cells numbers were observed. In the intranasal immunization group, CD4+ cells showed an obvious increase during the 4th-8th week after vaccination, and reached the highest level on the 6th week, CD8+ subsets had no obvious changes. CONCLUSION: CD4+ T cell cells may play a key role in immune response in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.