Contribution of GPR30 for 1,25 dihydroxyvitamin D3 protection in EAE.
Metab Brain Dis
; 27(1): 29-35, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-21994003
ABSTRACT
Previous studies have demonstrated that vitamin D3-mediated protection in EAE occurs only in females and is dependent on the presence of diestrus levels of 17ß-estradiol (E2). To evaluate the role of estrogen receptors in vitamin D3 treatment of EAE, we compared disease severity, CNS histopathology and immunological responses in vehicle and calcitrol (1,25 dihydroxyvitamin D3) treated WT C57BL/6 mice vs. GPR30 membrane estrogen receptor (MER) knockout mice with MOG-35-55 peptide-induced EAE. Our results demonstrated that vitamin D3-mediated prevention of clinical signs, CNS cellular lesions and demyelination observed in WT mice was abrogated in GPR30-KO mice with EAE. Regulatory effects of vitamin D3 treatment that were MER dependent included increased levels of IL-10 and IL-6 secreted by MOG peptide-reactive splenocytes and increased expression of CCL5, CCR1 & CCR3 in spleen tissue. These results demonstrate for the first time that the MER is a key contributor to the E2-dependent effects of vitamin D3-mediated protection in EAE.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Calcitriol
/
Receptores de Estrógenos
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Citoprotección
/
Receptores Acoplados a Proteínas G
/
Encefalomielitis Autoinmune Experimental
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Metab Brain Dis
Asunto de la revista:
CEREBRO
/
METABOLISMO
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos