[The correlation between expression level of femB and resistance phenotype of methicillin-resistant Staphylococcus aureus (MRSA)].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 42(5): 661-5, 2011 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-22007494
ABSTRACT
OBJECTIVE:
To investigate the correlation between the phenotype and expression level of femB of Staphylococcus aureus (MRSA), to discuss the mechanism of different phenotypes in methicillin-resistant Staphylococcus aureus.METHODS:
The minimal inhibitory concentrations (MICs) of oxacillin against 71 clinical isolates of Staphylococcus aureus were determined by agar dilution method according to NCCLS. The production of beta-lactamase was identified by Cefinase paper strip method. The isolation rate of beta-lactamase-producing strains was counted and the correlation between the resistance phenotype and isolation rate of beta-lactamase was analysed by statistics. Real time fluorescent quantitative PCR was used to quantify the mRNA expression of femB of non-beta-lactamase-producing strains.RESULTS:
The resistance rate of 71 Staphylococcus aureus to oxacillin was 66.20% (47/71), the isolation rate of beta-lactamase-producing MSSA strains was 58.3%,and that of strains of high- and low-level resistance to oxacillin were 63.15% and 55.56%. The standard curve was performed by series dilution of the heterogeneous resistant strain BB270, and the amount of femB-specific mRNA in strain BB270 was set to be 1. The calculated femB amounts in MSSA strains were from 0.4830-3.3636, while the amounts were from 0.4204-3.3636 in low-level MRSA strains, and 0.0718-16.0000 in high-level MRSA strains. There were no difference in the level of femB among MSSA, high-level MRSA and low-level MRSA.CONCLUSION:
The expression level of femB may not be related to the resistance of non-beta-lactamase-producting Staphylococcus aureus to methicillin.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oxacilina
/
Proteínas Bacterianas
/
Farmacorresistencia Microbiana
/
Staphylococcus aureus Resistente a Meticilina
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2011
Tipo del documento:
Article
País de afiliación:
China