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The aromatic peroxygenase from Marasmius rutola--a new enzyme for biosensor applications.
Yarman, Aysu; Gröbe, Glenn; Neumann, Bettina; Kinne, Mathias; Gajovic-Eichelmann, Nenad; Wollenberger, Ulla; Hofrichter, Martin; Ullrich, René; Scheibner, Katrin; Scheller, Frieder W.
Afiliación
  • Yarman A; Fraunhofer Institute for Biomedical Engineering IBMT, Potsdam, Germany.
Anal Bioanal Chem ; 402(1): 405-12, 2012 Jan.
Article en En | MEDLINE | ID: mdl-22038589
The aromatic peroxygenase (APO; EC 1.11.2.1) from the agraric basidomycete Marasmius rotula (MroAPO) immobilized at the chitosan-capped gold-nanoparticle-modified glassy carbon electrode displayed a pair of redox peaks with a midpoint potential of -278.5 mV vs. AgCl/AgCl (1 M KCl) for the Fe(2+)/Fe(3+) redox couple of the heme-thiolate-containing protein. MroAPO oxidizes aromatic substrates such as aniline, p-aminophenol, hydroquinone, resorcinol, catechol, and paracetamol by means of hydrogen peroxide. The substrate spectrum overlaps with those of cytochrome P450s and plant peroxidases which are relevant in environmental analysis and drug monitoring. In M. rotula peroxygenase-based enzyme electrodes, the signal is generated by the reduction of electrode-active reaction products (e.g., p-benzoquinone and p-quinoneimine) with electro-enzymatic recycling of the analyte. In these enzyme electrodes, the signal reflects the conversion of all substrates thus representing an overall parameter in complex media. The performance of these sensors and their further development are discussed.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Fúngicas / Técnicas Biosensibles / Enzimas Inmovilizadas / Marasmius / Oxigenasas de Función Mixta Idioma: En Revista: Anal Bioanal Chem Año: 2012 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Fúngicas / Técnicas Biosensibles / Enzimas Inmovilizadas / Marasmius / Oxigenasas de Función Mixta Idioma: En Revista: Anal Bioanal Chem Año: 2012 Tipo del documento: Article País de afiliación: Alemania