On the properties of calcium-induced permeability transition in neonatal heart mitochondria.
J Bioenerg Biomembr
; 43(6): 757-64, 2011 Dec.
Article
en En
| MEDLINE
| ID: mdl-22108703
ABSTRACT
Permeability transition was examined in heart mitochondria isolated from neonate rats. We found that these mitochondria were more susceptible to Ca(2+)-induced membrane leakiness than mitochondria from adult rats. In K(+) containing medium, at 25 °C, mitochondria were unable to accumulate Ca(2+). Conversely, in Na(+) containing medium, mitochondria accumulated effectively Ca(2+). At 15 °C mitochondria accumulated Ca(2+) regardless of the presence of K(+). Kinetics of Ca(2+) accumulation showed a similar Vmax as that of adult mitochondria. Lipid milieu of inner membrane contained more unsaturated fatty acids than adult mitochondria. Aconitase inhibition and high thiobarbituric acid-reactive substances (TBARS) indicate that oxidative stress caused mitochondrial damage. In addition, proteomics analysis showed that there is a considerable diminution of succinate dehydrogenase C and subunit 4 of cytochrome oxidase in neonate mitochondria. Our proposal is that dysfunction of the respiratory chain makes neonate mitochondria more susceptible to damage by oxidative stress.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Calcio
/
Estrés Oxidativo
/
Membranas Mitocondriales
/
Mitocondrias Cardíacas
Límite:
Animals
Idioma:
En
Revista:
J Bioenerg Biomembr
Año:
2011
Tipo del documento:
Article
País de afiliación:
México