[The purification and characterization of polysaccharides isolated from Ginkgo biloba and their in vitro antioxidant activities].
Zhong Yao Cai
; 34(12): 1950-3, 2011 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-22500437
ABSTRACT
OBJECTIVE:
To isolate and identify the polysaccharides of Ginkgo biloba and determine their antioxidant activities in vitro.METHODS:
Used hot extraction and alcohol precipitation to get the crude polysaccharides, removed protein with Sevag method, further purify by column chromatography packed with DEAE-52 and DEAE-Sepharose Fast Flow. The homogeneity and molecular weights were evaluated, configuration and monosaccharide composition were measured by IR and high performance ion chromatography analysis, the scavenging activities of GBPB-S on hydroxyl radical and DPPH were measured.RESULTS:
The molecular weights of GBPB-W and GBPB-S were 26 300 and 19 100, and both of them were composed of rhamnose, arabinose, galactose, glucose and mannose with the ratios of (3.48 8.473.73 1.76 1) and (5.34 5.37 5.27 11.68). GBPB-S had certain scavenging effect on hydroxyl radical and DPPH.CONCLUSION:
The polysaccharide isolated from Ginkgo biloba has a direct antioxidant activities in vitro.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Depuradores de Radicales Libres
/
Tecnología Farmacéutica
/
Ginkgo biloba
Idioma:
Zh
Revista:
Zhong Yao Cai
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2011
Tipo del documento:
Article
País de afiliación:
China