Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array.
J Nanosci Nanotechnol
; 12(6): 5004-8, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-22905567
ABSTRACT
We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
/
Bacterias
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Técnicas Biosensibles
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Recuento de Colonia Microbiana
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Nanotecnología
/
Nanoestructuras
Idioma:
En
Revista:
J Nanosci Nanotechnol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán