4C technology: protocols and data analysis.
Methods Enzymol
; 513: 89-112, 2012.
Article
en En
| MEDLINE
| ID: mdl-22929766
ABSTRACT
Chromosome conformation capture (3C) technology and its genome-wide derivatives have revolutionized our knowledge on chromatin folding and nuclear organization. 4C-seq Technology combines 3C principles with high-throughput sequencing (4C-seq) to enable for unbiased genome-wide screens for DNA contacts made by single genomic sites of interest. Here, we discuss in detail the design, application, and data analysis of 4C-seq experiments. Based on many hundreds of different 4C-seq experiments, we define criteria to assess data quality and show how different restriction enzymes and cross-linking conditions affect results. We describe in detail the mapping strategy of 4C-seq reads and show advanced strategies for data analysis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Cromatina
/
Estadística como Asunto
/
Mapeo Cromosómico
/
Análisis de Secuencia de ADN
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Methods Enzymol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Países Bajos