Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor.
Biotechnol Lett
; 34(12): 2307-15, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-22983716
ABSTRACT
The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was used in a pilot study to expand 60 million human embryonic stem cells to 708 million cells. Further improvements can be expected with optimisation of media flow rates throughout the run to better control the cellular microenvironment. High levels of pluripotency marker expression were maintained on the bioreactor, with 97.7 % of cells expressing SSEA-4 when harvested.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
/
Reactores Biológicos
/
Células Madre Embrionarias
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Biotechnol Lett
Año:
2012
Tipo del documento:
Article
País de afiliación:
Reino Unido