Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation.
Reproduction
; 145(5): 471-8, 2013 May.
Article
en En
| MEDLINE
| ID: mdl-23630331
ABSTRACT
The knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen-thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7-24âh from activation) by 2',7'-dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P<0.05 by ANOVA). In sperm-activated oocytes, an increase was observed between 15 and 19âh (P<0.05). Conversely, in parthenogenetically activated oocytes, we observed a decrease at 9âh (P<0.05). In sperm-activated oocytes, ROS fluctuated throughout the 24âh, presenting peaks around 7, 19, and 24âh (P<0.05), while in parthenogenetic activation, peaks were detected at 7, 11, and 17âh (P<0.05). In the present work, we found clear distinctive metabolic patterns between normal and parthenogenetic zygotes. Oxidative activity and ROS production are an integral part of bovine zygote behavior, and defining a temporal pattern of change may be linked with developmental competence.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Partenogénesis
/
Interacciones Espermatozoide-Óvulo
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Cigoto
/
Bovinos
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Especies Reactivas de Oxígeno
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Ectogénesis
Límite:
Animals
Idioma:
En
Revista:
Reproduction
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Argentina