Genetic diversity in exon 2 of the major histocompatibility complex class II DQB1 locus in Nigerian goats.
Biochem Genet
; 51(11-12): 954-66, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-23835918
ABSTRACT
The DQB1 locus is located in the major histocompatibility complex (MHC) class II region and involved in immune response. We identified 20 polymorphic sites in a 228 bp fragment of exon 2, one of the most critical regions of the MHC DQB1 gene, in 60 Nigerian goats. Four sites are located in the peptide binding region, and 10 amino acid substitutions are peculiar to Nigerian goats, compared with published sequences. A significantly higher ratio of nonsynonymous/synonymous substitutions (dN/dS) suggests that allelic sequence evolution is driven by balancing selection (P < 0.01). In silico functional analysis using PANTHER predicted that substitution P56R, with a subPSEC score of -4.00629 (Pdeleterious = 0.73229), is harmful to protein function. The phylogenetic tree from consensus sequences placed the two northern breeds closer to each other than either was to the southern goats. This first report of sequence diversity at the DQB1 locus for any African goat breed may be useful in the search for disease-resistant genotypes.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Variación Genética
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Cabras
/
Exones
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Genes MHC Clase II
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Complejo Mayor de Histocompatibilidad
Tipo de estudio:
Prognostic_studies
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
Biochem Genet
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos