Characterization of regulatory mechanism of Poncirus trifoliata microRNAs on their target genes with an integrated strategy of newly developed PPM-RACE and RLM-RACE.

Shangguan, Lingfei; Song, Changnian; Han, Jian; Leng, Xiangpeng; Kibet, Korir Nicholas; Mu, Qian; Kayesh, Emrul; Fang, Jinggui

Shangguan, Lingfei; Song, Changnian; Han, Jian; Leng, Xiangpeng; Kibet, Korir Nicholas; Mu, Qian; Kayesh, Emrul; Fang, Jinggui.

Afiliación

Shangguan L; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: shangguanlf@gmail.com.
Song C; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: songchangnian@njau.edu.cn.
Han J; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: hanjian@njau.edu.cn.
Leng X; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2010104015@njau.edu.cn.
Kibet KN; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2009204036@njau.edu.cn.
Mu Q; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2010104017@njau.edu.cn.
Kayesh E; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: e.kayesh@yahoo.com.
Fang J; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: fanggg@njau.edu.cn.

Gene ; 535(1): 42-52, 2014 Feb 01.

Article en En | MEDLINE | ID: mdl-24275346

Asunto(s)

Genes de Plantas; MicroARNs/genética; Poncirus/genética; Regulación de la Expresión Génica de las Plantas; ARN de Planta/genética; Reacción en Cadena en Tiempo Real de la Polimerasa

Palabras clave

NCBI; National Center for Biotechnology Information; PAP; PCR; PPM-RACE; PPM-RACE and RLM-RACE; Poncirus trifoliata; RACE; RLM-5' RACE; RLM-RACE; RNA; RNA ligase-mediated 5' rapid amplification of cDNA ends; RT-PCR; Target mRNA; cDNA; complementary DNA; miRNA; miRNA-mediated 3' and 5' cleavage products; microRNA; poly (A) polymerase; poly (A) polymerase-mediated 3' RACE and RNA ligase-mediated 5' RACE; polymerase chain reaction; qRT-PCR; quantitative RT-PCR; rapid amplification of cDNA ends; reverse transcription polymerase chain reaction; ribonucleic acid

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Genes de Plantas / Poncirus / MicroARNs Idioma: En Revista: Gene Año: 2014 Tipo del documento: Article

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