Structure elucidation of DNA-protein crosslinks by using reductive desulfurization and liquid chromatography-tandem mass spectrometry.

Wickramaratne, Susith; Tretyakova, Natalia Y

Wickramaratne, Susith; Tretyakova, Natalia Y.

Afiliación

Wickramaratne S; University of Minnesota Masonic Cancer Center and the Department of Chemistry, 2231 6th Street SE, Room 2-220 CCRB, Minneapolis, MN 55455 (USA).

Chembiochem ; 15(3): 353-5, 2014 Feb 10.

Article en En | MEDLINE | ID: mdl-24436288

ABSTRACT

ABSTRACT

Easier with ethyl Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT-DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl-DNA adducts, which can be readily characterized by LC-MSn.

Asunto(s)

Aductos de ADN/química; ADN/química; O(6)-Metilguanina-ADN Metiltransferasa/química; Azufre/química; Cromatografía Líquida de Alta Presión; Cisteína/química; ADN/metabolismo; Guanina/química; O(6)-Metilguanina-ADN Metiltransferasa/metabolismo; Oxidación-Reducción; Espectrometría de Masa por Ionización de Electrospray

Palabras clave

DNA-protein crosslinks; O6-alkylguanine DNA alkyltransferase; desulfurization; labile and nonlabile DNA adducts; liquid chromatography-tandem mass spectrometry

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Azufre / ADN / Aductos de ADN / O(6)-Metilguanina-ADN Metiltransferasa Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2014 Tipo del documento: Article

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