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A synthetic luciferin improves bioluminescence imaging in live mice.
Evans, Melanie S; Chaurette, Joanna P; Adams, Spencer T; Reddy, Gadarla R; Paley, Miranda A; Aronin, Neil; Prescher, Jennifer A; Miller, Stephen C.
Afiliación
  • Evans MS; 1] Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, USA. [2].
  • Chaurette JP; 1] Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [2] RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [3].
  • Adams ST; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
  • Reddy GR; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
  • Paley MA; Department of Chemistry, University of California, Irvine, Irvine, California, USA.
  • Aronin N; 1] Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [2] RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
  • Prescher JA; 1] Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, USA. [2] Department of Chemistry, University of California, Irvine, Irvine, California, USA.
  • Miller SC; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Nat Methods ; 11(4): 393-5, 2014 Apr.
Article en En | MEDLINE | ID: mdl-24509630
ABSTRACT
Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Benzotiazoles / Neuroimagen / Mediciones Luminiscentes Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2014 Tipo del documento: Article
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Benzotiazoles / Neuroimagen / Mediciones Luminiscentes Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2014 Tipo del documento: Article