Your browser doesn't support javascript.
loading
Wnt-promoted Isl1 expression through a novel TCF/LEF1 binding site and H3K9 acetylation in early stages of cardiomyocyte differentiation of P19CL6 cells.
Lu, Huafei; Li, Yanming; Wang, Yang; Liu, Yinan; Wang, Weiping; Jia, Zhuqing; Chen, Ping; Ma, Kangtao; Zhou, Chunyan.
Afiliación
  • Lu H; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education of China, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
Mol Cell Biochem ; 391(1-2): 183-92, 2014 Jun.
Article en En | MEDLINE | ID: mdl-24610003
ABSTRACT
Islet 1 (ISL1), a marker of second heart field progenitors, plays a crucial role in cardiomyocyte differentiation and proliferation. However, little is known about transcriptional regulating mechanisms on Isl1 gene expression. Recent studies have demonstrated that Wnt/ß-catenin signaling regulates Isl1 expression during heart development. However, the detailed mechanisms still remain unclear. In the present study performed during differentiation of P19CL6 into cardiomyocytes, we explored the underlying regulating mechanisms on Wnt/ß-catenin-mediated Isl1 expression after we first confirmed that Wnt/ß-catenin signaling promoted cardiomyocyte differentiation partly through Isl1 activation. We found a novel TCF/LEF1 binding site that was located 2300 bp upstream of the Isl1 ATG. Furthermore, Wnt/ß-catenin signaling upregulated histone H3K9 acetylation on TCF/LEF1 binding sites on the Isl1 promoter, resulting in upregulation of Isl1 expression. This Wnt-mediated H3K9 acetylation on the Isl1 promoter was modulated by the acetyltransferase CREB-binding protein (CBP), instead of p300, through interaction with ß-catenin. Collectively, these results suggest that in early stages of cardiomyocyte differentiation Wnt/ß-catenin signaling promotes Isl1 expression via two ways a novel TCF/LEF1 binding site and H3K9 acetylation conducted by CBP on the Isl1 promoter. To our knowledge, this is the first study reporting Wnt/ß-catenin-regulated H3K9 acetylation on promoters of its target genes. And this study gives new insights into transcriptional regulating mechanisms of Wnt-mediated Isl1 expression during cardiomyocyte differentiation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Factores de Transcripción / Histonas / Diferenciación Celular / Miocitos Cardíacos / Proteínas Wnt / Factor de Unión 1 al Potenciador Linfoide / Proteínas con Homeodominio LIM Límite: Animals Idioma: En Revista: Mol Cell Biochem Año: 2014 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Factores de Transcripción / Histonas / Diferenciación Celular / Miocitos Cardíacos / Proteínas Wnt / Factor de Unión 1 al Potenciador Linfoide / Proteínas con Homeodominio LIM Límite: Animals Idioma: En Revista: Mol Cell Biochem Año: 2014 Tipo del documento: Article País de afiliación: China