Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity.
FEBS Lett
; 242(1): 161-6, 1988 Dec 19.
Article
en En
| MEDLINE
| ID: mdl-2462511
ABSTRACT
Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH2-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10(-7)-10(-8) M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Peces Planos
/
Venenos de los Peces
/
Ionóforos
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1988
Tipo del documento:
Article