New insights on plant cell elongation: a role for acetylcholine.
Int J Mol Sci
; 15(3): 4565-82, 2014 Mar 17.
Article
en En
| MEDLINE
| ID: mdl-24642879
ABSTRACT
We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Acetilcolina
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Solanum lycopersicum
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Tamaño de la Célula
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Células Vegetales
Idioma:
En
Revista:
Int J Mol Sci
Año:
2014
Tipo del documento:
Article
País de afiliación:
Italia