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Phase maintenance in a rhythmic motor pattern during temperature changes in vivo.
Soofi, Wafa; Goeritz, Marie L; Kispersky, Tilman J; Prinz, Astrid A; Marder, Eve; Stein, Wolfgang.
Afiliación
  • Soofi W; Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia;
  • Goeritz ML; Department of Biology and Volen Center, Brandeis University, Waltham, Massachusetts;
  • Kispersky TJ; Department of Biology and Volen Center, Brandeis University, Waltham, Massachusetts;
  • Prinz AA; Department of Biology, Emory University, Atlanta, Georgia;
  • Marder E; Department of Biology and Volen Center, Brandeis University, Waltham, Massachusetts;
  • Stein W; School of Biological Sciences, Illinois State University, Normal, Illinois; and Institute for Neurobiology, Ulm University, Ulm, Germany wstein@neurobiologie.de.
J Neurophysiol ; 111(12): 2603-13, 2014 Jun 15.
Article en En | MEDLINE | ID: mdl-24671541
ABSTRACT
Central-pattern-generating neural circuits function reliably throughout an animal's life, despite constant molecular turnover and environmental perturbations. Fluctuations in temperature pose a problem to the nervous systems of poikilotherms because their body temperature follows the ambient temperature, thus affecting the temperature-dependent dynamics of various subcellular components that constitute neuronal circuits. In the crustacean stomatogastric nervous system, the pyloric circuit produces a triphasic rhythm comprising the output of the pyloric dilator, lateral pyloric, and pyloric constrictor neurons. In vitro, the phase relationships of these neurons are maintained over a fourfold change in pyloric frequency as temperature increases from 7°C to 23°C. To determine whether these temperature effects are also found in intact crabs, in the presence of sensory feedback and neuromodulator-rich environments, we measured the temperature dependence of the pyloric frequency and phases in vivo by implanting extracellular electrodes into Cancer borealis and Cancer pagurus and shifting tank water temperature from 11°C to 26°C. Pyloric frequency in the intact crab increased significantly with temperature (Q10 = 2-2.5), while pyloric phases were generally conserved. For a subset of the C. borealis experiments, animals were subsequently dissected and the stomatogastric ganglion subjected to a similar temperature ramp in vitro. We found that the maximal frequency attained at high temperatures in vivo is lower than it is under in vitro conditions. Our results demonstrate that, over a wide temperature range, the phases of the pyloric rhythm in vivo are generally preserved, but that the frequency range is more restricted than it is in vitro.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Temperatura / Relojes Biológicos / Ganglios de Invertebrados / Braquiuros / Actividad Motora Límite: Animals Idioma: En Revista: J Neurophysiol Año: 2014 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Temperatura / Relojes Biológicos / Ganglios de Invertebrados / Braquiuros / Actividad Motora Límite: Animals Idioma: En Revista: J Neurophysiol Año: 2014 Tipo del documento: Article