Biophysical optimization of a therapeutic protein by nonstandard mutagenesis: studies of an iodo-insulin derivative.

Pandyarajan, Vijay; Phillips, Nelson B; Cox, Gabriela P; Yang, Yanwu; Whittaker, Jonathan; Ismail-Beigi, Faramarz; Weiss, Michael A

Pandyarajan, Vijay; Phillips, Nelson B; Cox, Gabriela P; Yang, Yanwu; Whittaker, Jonathan; Ismail-Beigi, Faramarz; Weiss, Michael A.

Afiliación

Pandyarajan V; From the Departments of Biochemistry.
Phillips NB; From the Departments of Biochemistry.
Cox GP; Physiology and Biophysics.
Yang Y; From the Departments of Biochemistry.
Whittaker J; From the Departments of Biochemistry.
Ismail-Beigi F; Physiology and Biophysics, Medicine, and.
Weiss MA; From the Departments of Biochemistry, Medicine, and Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106 michael.weiss@case.edu.

J Biol Chem ; 289(34): 23367-81, 2014 Aug 22.

Article en En | MEDLINE | ID: mdl-24993826

Asunto(s)

Insulina/análogos & derivados; Mutagénesis; Animales; Fenómenos Biofísicos; Dicroismo Circular; Insulina/química; Insulina/genética; Insulina/farmacocinética; Masculino; Resonancia Magnética Nuclear Biomolecular; Ingeniería de Proteínas; Ratas; Ratas Endogámicas Lew; Receptor de Insulina/metabolismo; Espectrofotometría Ultravioleta

Palabras clave

Diabetes; Hormone; Insulin; Nonstandard Mutagenesis; Nuclear Magnetic Resonance (NMR); Protein Allostery; Protein Design; Protein Stability

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Mutagénesis / Insulina Límite: Animals Idioma: En Revista: J Biol Chem Año: 2014 Tipo del documento: Article

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