Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages.
Appl Environ Microbiol
; 80(18): 5623-35, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-25002431
ABSTRACT
In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach:
(i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bacteriófagos
/
Proteínas Virales
/
Virión
/
ADN Viral
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Lactobacillus delbrueckii
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Especificidad del Huésped
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Irlanda