MIRA analysis of RARß2 gene methylation in DNA circulating in the blood in lung cancer.
Bull Exp Biol Med
; 157(4): 516-9, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-25110096
ABSTRACT
Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment efficiency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfite conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for RARß2 tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN de Neoplasias
/
Biomarcadores de Tumor
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Receptores de Ácido Retinoico
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Carcinoma de Pulmón de Células no Pequeñas
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Epigénesis Genética
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Neoplasias Pulmonares
Tipo de estudio:
Observational_studies
Límite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Bull Exp Biol Med
Año:
2014
Tipo del documento:
Article
País de afiliación:
Rusia