Isolation and purification of two antioxidant peptides from alcalase hydrolysate of Arca subcrenata.

ABSTRACT

OBJECTIVE: To investigate the constituents with antioxidant activities from alcalase hydrolysate of Arca subcrenata. METHODS: The consecutive chromatographic methods were employed,including ion-exchange chromatography, gel filtration chromatography, and reverse phase high-performance liquid chromatography (RP-HPLC). The amino acid sequences of the purified antioxidant peptides were determined by automated Edman degradation. RESULTS: Under the guidance of the assay of scavenging free radicals, two peptides with antioxidant activities, termed as A-Bg1 and A-Bh, were isolated and purified from the alcalase hydrolysate of Arca subcrenata. CONCLUSION: Constituents from the hydrolysate of Arca subcrenata might be a new potential resource of antioxidants.