Comparison of monocyte separation methods using flow cytometric analysis.
J Immunol Methods
; 125(1-2): 41-7, 1989 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-2575116
ABSTRACT
We isolated normal, nonactivated human monocytes from peripheral blood by four different methods:
(1) rosetting with sheep erythrocytes pretreated with 2-aminoethylisothiouronium bromide hydrobromide (AET) followed by monoclonal antibody (OKT3 (CD3), B1 (CD19), Leu7, Leu11 (CD16] and complement treatment; (2) adherence to gelatin/plasma-coated flasks; (3) adherence to plastic dishes; and (4) separation by the Sepracell technique. We monitored these monocyte separations by determining cell recoveries, OKT4A+ lymphocyte contamination, monocyte binding to human immunodeficiency virus (HIV), number of non-specific esterase-positive cells, and proportion of mononuclear cells reactive with a battery of monoclonal antibodies specific for monocytes. Our results indicate that of the four methods compared, adherence to gelatin/plasma-coated flasks produced the highest purity, recovery, and satisfactory binding to HIV with the fewest contaminating CD4+ T cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Monocitos
/
Separación Celular
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1989
Tipo del documento:
Article