A Spatial Model of Insulin-Granule Dynamics in Pancreatic ß-Cells.
Traffic
; 16(8): 797-813, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-25809669
ABSTRACT
Insulin secretion from pancreatic ß-cells in response to sudden glucose stimulation is biphasic. Prolonged secretion in vivo requires synthesis, delivery to the plasma membrane (PM) and exocytosis of insulin secretory granules (SGs). Here, we provide the first agent-based space-resolved model for SG dynamics in pancreatic ß-cells. Using recent experimental data, we consider a single ß-cell with identical SGs moving on a phenomenologically represented cytoskeleton network. A single exocytotic machinery mediates SG exocytosis on the PM. This novel model reproduces the measured spatial organization of SGs and insulin secretion patterns under different stimulation protocols. It proposes that the insulin potentiation effect and the rising second-phase secretion are mainly due to the increasing number of docking sites on the PM. Furthermore, it shows that 6 min after glucose stimulation, the 'newcomer' SGs are recruited from a region within less than 600 nm from the PM.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Vesículas Secretoras
/
Células Secretoras de Insulina
/
Insulina
/
Modelos Biológicos
Tipo de estudio:
Qualitative_research
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Traffic
Asunto de la revista:
FISIOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania