Stability of urine specimens stored with and without preservatives at room temperature and on ice prior to urinalysis.
Clin Biochem
; 48(13-14): 919-22, 2015 Sep.
Article
en En
| MEDLINE
| ID: mdl-26025772
ABSTRACT
OBJECTIVES:
Laboratories determine the most appropriate approach for the collection and transport of urine specimens. We investigated the effect of a chlorhexidine-based preservative tube on sample stability, compared the results of refrigerated polystyrene tubes with no additives, and investigated the effect of temperature on the performance of preservative tubes. DESIGN ANDMETHODS:
Fresh urine specimen (n=48) aliquots in BD Vacutainer® Plus Urinalysis Preservative Tubes and polystyrene tubes were analyzed on an Iris Diagnostics iQ200. Samples in polystyrene tubes were refrigerated for 4 and 8h. Four aliquots in preservative tubes were kept at room temperature for 4, 8, 24, and 72h, while two aliquots were kept on ice for 4 and 8h.RESULTS:
There was good agreement for all chemistry and microscopy parameters with the exceptions of white blood cells (WBCs) at 24 and 72h and red blood cells (RBCs) at 72h. Preservative tubes on ice showed a significant decrease in concordance of WBCs and calcium oxalate (CaOx) parameters compared with the results at room temperature. Results of refrigerated polystyrene tubes showed good agreement with the exceptions of WBC clumps and amorphous crystal at 8h.CONCLUSIONS:
A chlorhexidine-containing preservative tube seems advantageous for urine sample transport from outside healthcare services. A preservative tube offers comparable results with urine samples kept in a refrigerator for 4-8h for the majority of parameters. Keeping samples at room temperature is recommended when preservative tubes are used because ice produces a negative effect on WBCs and CaOx.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Conservadores Farmacéuticos
/
Manejo de Especímenes
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Temperatura
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Criopreservación
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Urinálisis
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Hielo
Límite:
Humans
Idioma:
En
Revista:
Clin Biochem
Año:
2015
Tipo del documento:
Article
País de afiliación:
Turquía