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Long-Lived Plasma Cells Are Contained within the CD19(-)CD38(hi)CD138(+) Subset in Human Bone Marrow.
Halliley, Jessica L; Tipton, Christopher M; Liesveld, Jane; Rosenberg, Alexander F; Darce, Jaime; Gregoretti, Ivan V; Popova, Lana; Kaminiski, Denise; Fucile, Christopher F; Albizua, Igor; Kyu, Shuya; Chiang, Kuang-Yueh; Bradley, Kyle T; Burack, Richard; Slifka, Mark; Hammarlund, Erika; Wu, Hao; Zhao, Liping; Walsh, Edward E; Falsey, Ann R; Randall, Troy D; Cheung, Wan Cheung; Sanz, Iñaki; Lee, F Eun-Hyung.
Afiliación
  • Halliley JL; Divisions of Pulmonary, Allergy, & Critical Care Medicine, Emory University, Atlanta, GA 30322, USA; Pulmonary & Critical Care Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA.
  • Tipton CM; Division of Rheumatology, Emory University, Atlanta, GA 30322, USA; Lowance Center for Human Immunology in the Departments of Medicine and Pediatrics, Emory University, Atlanta, GA 30322, USA.
  • Liesveld J; Divisions of Hematology/Oncology/James P. Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, NY 14642, USA.
  • Rosenberg AF; Division of Allergy, Immunology, and Rheumatology, University of Rochester Medical Center, Rochester, NY 14642, USA.
  • Darce J; Cell Signaling Technology, Inc., Danvers, MA 01923, USA.
  • Gregoretti IV; Cell Signaling Technology, Inc., Danvers, MA 01923, USA.
  • Popova L; Cell Signaling Technology, Inc., Danvers, MA 01923, USA.
  • Kaminiski D; Division of Allergy, Immunology, and Rheumatology, University of Rochester Medical Center, Rochester, NY 14642, USA.
  • Fucile CF; Division of Allergy, Immunology, and Rheumatology, University of Rochester Medical Center, Rochester, NY 14642, USA.
  • Albizua I; Divisions of Pulmonary, Allergy, & Critical Care Medicine, Emory University, Atlanta, GA 30322, USA.
  • Kyu S; Divisions of Pulmonary, Allergy, & Critical Care Medicine, Emory University, Atlanta, GA 30322, USA.
  • Chiang KY; Department of Pediatrics, Aflac Cancer and Blood Disorders Center, Emory University, Atlanta, GA 30322, USA.
  • Bradley KT; Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA 30322, USA.
  • Burack R; Department of Pathology, University of Rochester Medical Center, Rochester, NY 14642, USA.
  • Slifka M; Oregon Health & Sciences University, Beaverton, OR 97006, USA.
  • Hammarlund E; Oregon Health & Sciences University, Beaverton, OR 97006, USA.
  • Wu H; Department of Biostatistics and Bioinformatics, Emory University, Atlanta, GA 30322, USA.
  • Zhao L; Department of Biostatistics and Bioinformatics, Emory University, Atlanta, GA 30322, USA.
  • Walsh EE; Division of Infectious Diseases, University of Rochester Medical Center & Rochester General Hospital, Rochester, NY 14621, USA.
  • Falsey AR; Division of Infectious Diseases, University of Rochester Medical Center & Rochester General Hospital, Rochester, NY 14621, USA.
  • Randall TD; Division of Clinical Immunology and Rheumatology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
  • Cheung WC; Cell Signaling Technology, Inc., Danvers, MA 01923, USA.
  • Sanz I; Division of Rheumatology, Emory University, Atlanta, GA 30322, USA; Lowance Center for Human Immunology in the Departments of Medicine and Pediatrics, Emory University, Atlanta, GA 30322, USA.
  • Lee FE; Divisions of Pulmonary, Allergy, & Critical Care Medicine, Emory University, Atlanta, GA 30322, USA; Lowance Center for Human Immunology in the Departments of Medicine and Pediatrics, Emory University, Atlanta, GA 30322, USA. Electronic address: f.e.lee@emory.edu.
Immunity ; 43(1): 132-45, 2015 Jul 21.
Article en En | MEDLINE | ID: mdl-26187412
ABSTRACT
Antibody responses to viral infections are sustained for decades by long-lived plasma cells (LLPCs). However, LLPCs have yet to be characterized in humans. Here we used CD19, CD38, and CD138 to identify four PC subsets in human bone marrow (BM). We found that the CD19(-)CD38(hi)CD138(+) subset was morphologically distinct, differentially expressed PC-associated genes, and exclusively contained PCs specific for viral antigens to which the subjects had not been exposed for more than 40 years. Protein sequences of measles- and mumps-specific circulating antibodies were encoded for by CD19(-)CD38(hi)CD138(+) PCs in the BM. Finally, we found that CD19(-)CD38(hi)CD138(+) PCs had a distinct RNA transcriptome signature and human immunoglobulin heavy chain (VH) repertoire that was relatively uncoupled from other BM PC subsets and probably represents the B cell response's "historical record" of antigenic exposure. Thus, our studies define human LLPCs and provide a mechanism for the life-long maintenance of anti-viral antibodies in the serum.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Plasmáticas / Células de la Médula Ósea / Virus del Sarampión / Anticuerpos Antivirales / Virus de la Parotiditis Tipo de estudio: Prognostic_studies Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: Immunity Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Plasmáticas / Células de la Médula Ósea / Virus del Sarampión / Anticuerpos Antivirales / Virus de la Parotiditis Tipo de estudio: Prognostic_studies Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: Immunity Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos