Ebselen induces mitochondrial permeability transition because of its interaction with adenine nucleotide translocase.
Life Sci
; 139: 108-13, 2015 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-26316446
ABSTRACT
AIMS:
Mitochondrial permeability transition is a process established through massive Ca(2+) load in addition to an inducer reagent. Ebselen (Ebs), an antioxidant seleno compound, has been introduced as a reagent which inhibits mitochondrial dysfunction induced by permeability transition. Paradoxically enough, it has been shown that Ebs may also be able to induce the opening of the mitochondrial non-selective pores. This study was performed with the purpose of establishing the membrane system involved in Ebs-induced pore opening. MAINMETHODS:
Permeability transition was appraised by analyzing the following i) matrix Ca(2+) release, and mitochondrial swelling, ii) efflux of cytochrome c, and iii) the inhibition of superoxide dismutase. All of these adverse reactions were inhibited by N-ethylmaleimide and cyclosporin A. KEYFINDINGS:
At concentrations from 5 to 20 µM, we found that Ebs induces non-specific membrane permeability. Remarkably, Ebs blocks the binding of the fluorescent reagent eosin-5-maleimide to the thiol groups of the adenine nucleotide translocase.SIGNIFICANCE:
Based on the above, it is tempting to hypothesize that Ebs induces pore opening through its binding to the ADP/ATP carrier.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Permeabilidad
/
Azoles
/
Translocasas Mitocondriales de ADP y ATP
/
Compuestos de Organoselenio
/
Mitocondrias
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Dilatación Mitocondrial
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Antioxidantes
Límite:
Animals
Idioma:
En
Revista:
Life Sci
Año:
2015
Tipo del documento:
Article