Imaging organelle transport in primary hippocampal neurons treated with amyloid-ß oligomers.
Methods Cell Biol
; 131: 425-51, 2016.
Article
en En
| MEDLINE
| ID: mdl-26794527
ABSTRACT
We describe a strategy for fluorescent imaging of organelle transport in primary hippocampal neurons treated with amyloid-ß (Aß) peptides that cause Alzheimer's disease (AD). This method enables careful, rigorous analyses of axonal transport defects, which are implicated in AD and other neurodegenerative diseases. Moreover, we present and emphasize guidelines for investigating Aß-induced mechanisms of axonal transport disruption in the absence of nonspecific, irreversible cellular toxicity. This approach should be accessible to most laboratories equipped with cell culture facilities and a standard fluorescent microscope and may be adapted to other cell types.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Axones
/
Transporte Axonal
/
Péptidos beta-Amiloides
/
Factor Neurotrófico Derivado del Encéfalo
/
Hipocampo
Límite:
Animals
Idioma:
En
Revista:
Methods Cell Biol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Canadá