Sequencing ebola and marburg viruses genomes using microarrays.
J Med Virol
; 88(8): 1303-8, 2016 08.
Article
en En
| MEDLINE
| ID: mdl-26822839
ABSTRACT
Periodic outbreaks of Ebola and Marburg hemorrhagic fevers have occurred in Africa over the past four decades with case fatality rates reaching as high as 90%. The latest Ebola outbreak in West Africa in 2014 raised concerns that these infections can spread across continents and pose serious health risks. Early and accurate identification of the causative agents is necessary to contain outbreaks. In this report, we describe sequencing-by-hybridization (SBH) technique using high density microarrays to identify Ebola and Marburg viruses. The microarrays were designed to interrogate the sequences of entire viral genomes, and were evaluated with three species of Ebolavirus (Reston, Sudan, and Zaire), and three strains of Marburgvirus (Angola, Musoke, and Ravn). The results showed that the consensus sequences generated with four or more hybridizations had 92.1-98.9% accuracy over 95-99% of the genomes. Additionally, with SBH microarrays it was possible to distinguish between different strains of the Lake Victoria Marburgvirus. J. Med. Virol. 881303-1308, 2016. © 2016 Wiley Periodicals, Inc.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ebolavirus
/
Secuenciación de Nucleótidos de Alto Rendimiento
/
Marburgvirus
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Hibridación de Ácido Nucleico
Límite:
Animals
/
Humans
País/Región como asunto:
Africa
Idioma:
En
Revista:
J Med Virol
Año:
2016
Tipo del documento:
Article