[New osmo-regulational promoters in the industrial yeast].

OBJECTIVE: The work was aimed at selecting osmo-regulated prompters possessing excellent performance for further research of the industrial yeast Candida glycerinogenes. METHODS: Promoters PCgPGI, PCgTPI, PCgZWF, PCgSTL1, PCgSTL2 and PCgSTL3 were amplified by PCR and their bioinformatics analysis of stress response elements (STREs) were conducted. We constructed integrative plasmids containing 5.8S rDNA, a fluorescence protein gene gfp and a promoter PCgPGI, PCgTPI, PCgZWF, PCgSTL1, PCgSTL2 or PCgSTL3. The promoters' activities and osmo-regulations were compared according to the results of fluorescence and qRT-PCR. RESULTS: PCgSTL3 had more STREs, higher transcription level, lager gfp expression and it was more sensitive to stress. CONCLUSION: PCgSTL3 is an excellent induced promoter responding to hyperosmotic stress. Controlled expression of target genes can be realized using PCgSTL3 in the industrial yeast.