Selective solid-phase extraction of catecholamines from plasma using nanofibers doped with crown ether and their quantitation by HPLC with electrochemical detection.
Anal Bioanal Chem
; 408(18): 4987-94, 2016 Jul.
Article
en En
| MEDLINE
| ID: mdl-27173391
ABSTRACT
A simple and convenient preparation method has been developed for polymeric crown ethers containing the dibenzo-18-crown-6 subunit and electrospun composite nanofibers composed of polymeric crown ethers with polystyrene (PCE-PS). Furthermore, this composite nanofiber was used as a sorbent for selective extraction of plasma catecholamines, viz. dopamine (DA), norepinephrine (NE), and epinephrine (E). After the protein deposition of the plasma sample, the supernatant was adjusted to neutral pH with buffer solution. Then, the mixture was loaded and pushed through a column packed with composite nanofiber sorbent, and the analytes were eluted with 50 µL of acetic acid. The effectiveness of the plasma sample cleanup method was verified by high-performance liquid chromatography with electrochemical detection (HPLC-ECD). The PCE-PS packed column may prove clinically useful, as it provides a convenient and selective method for evaluation of human plasma catecholamines. Graphical Abstract Scheme showing the selective packed fiber SPE of catecholamines from spiked plasma using nanofibers doped with ploymeric crown ether.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Catecolaminas
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Cromatografía Líquida de Alta Presión
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Éteres Corona
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Electroquímica
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Extracción en Fase Sólida
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Nanofibras
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2016
Tipo del documento:
Article
País de afiliación:
China