[Using 9-color Flow Cytometry Immunophenotyping to Detect Activation and Apoptosis of Human TCR Vß Lymphocyte Subpopulations in Peripheral Blood Samples].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 47(2): 262-6, 2016 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-27263307
ABSTRACT
OBJECTIVE:
To establish an assay using 9-color flow cytometry immunophenotyping to detect activation and apoptosis of human TCR Vß lymphocyte subpopulations in peripheral blood samples.METHODS:
We used 5 antibodies (CD3, CD4, CD8, CD95, CD69), phospholipids binding proteins Annexin V, TCR Vß Repertoire Kit and nucleus dye DAPI to establish a 9-color flow cytometry assay. Peripheral blood samples were taken from eight healthy people for test of antibodies and determination of optimal PMT and staining method (single-stained vs stained with all but one antibody).RESULTS:
Appropriate detecting voltage, antibody concentration and compensation methods were determined. The distribution of TCR Vß subgroup in our samples was consistent with the TCR Vß Repertoire Kit instruction and other published literature.CONCLUSION:
We have established a effective easy using 9-color flow cytometry immunophenotyping to detect human TCR Vß lymphocyte subpopulations in peripheral blood samples.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Activación de Linfocitos
/
Inmunofenotipificación
/
Subgrupos Linfocitarios
/
Apoptosis
/
Citometría de Flujo
Límite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2016
Tipo del documento:
Article