A genetic assay for gene essentiality in Clostridium.

Walker, David J F; Heap, John T; Winzer, Klaus; Minton, Nigel P

Walker, David J F; Heap, John T; Winzer, Klaus; Minton, Nigel P.

Afiliación

Walker DJF; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Heap JT; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Winzer K; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Minton NP; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom; Nottingham Digestive Disease Centre, NIHR Biomedical Research Unit, The Universi

Anaerobe ; 42: 40-43, 2016 Dec.

Article en En | MEDLINE | ID: mdl-27487328

ABSTRACT

ABSTRACT

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.

Asunto(s)

Bioensayo; Clostridioides difficile/genética; Genes Esenciales; Ingeniería Genética/métodos; Metionina Adenosiltransferasa/genética; Triptófano-ARNt Ligasa/genética; Secuencia de Aminoácidos; Secuencia de Bases; Clonación Molecular; Duplicación de Gen; Expresión Génica

Palabras clave

ClosTron; Clostridium difficile; Gene essentiality; Merodiploid; Therapeutic target

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Triptófano-ARNt Ligasa / Bioensayo / Ingeniería Genética / Clostridioides difficile / Genes Esenciales / Metionina Adenosiltransferasa Idioma: En Revista: Anaerobe Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido

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