Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.

Camattari, Andrea; Goh, Amelia; Yip, Lian Yee; Tan, Andy Hee Meng; Ng, Sze Wai; Tran, Anthony; Liu, Gaowen; Liachko, Ivan; Dunham, Maitreya J; Rancati, Giulia

Camattari, Andrea; Goh, Amelia; Yip, Lian Yee; Tan, Andy Hee Meng; Ng, Sze Wai; Tran, Anthony; Liu, Gaowen; Liachko, Ivan; Dunham, Maitreya J; Rancati, Giulia.

Afiliación

Camattari A; Bioprocessing Technology Institute (A-STAR), 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore. andrea_camattari@bti.a-star.edu.sg.
Goh A; Bioprocessing Technology Institute (A-STAR), 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Yip LY; Bioprocessing Technology Institute (A-STAR), 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Tan AH; Bioprocessing Technology Institute (A-STAR), 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Ng SW; Bioprocessing Technology Institute (A-STAR), 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Tran A; Institute of Medical Biology (A-STAR), 8a Biomedical Grove, #06-06, Singapore, 138648, Singapore.
Liu G; Institute of Medical Biology (A-STAR), 8a Biomedical Grove, #06-06, Singapore, 138648, Singapore.
Liachko I; Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Dunham MJ; Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Rancati G; Institute of Medical Biology (A-STAR), 8a Biomedical Grove, #06-06, Singapore, 138648, Singapore.

Microb Cell Fact ; 15(1): 139, 2016 Aug 11.

Article en En | MEDLINE | ID: mdl-27515025

Asunto(s)

Vectores Genéticos; Pichia/genética; Plásmidos; Proteínas Recombinantes/biosíntesis; Recombinación Genética; Clonación Molecular; Regulación Fúngica de la Expresión Génica; Metanol/metabolismo; Pichia/metabolismo; Regiones Promotoras Genéticas; Procesamiento Proteico-Postraduccional; Biología Sintética/métodos

Palabras clave

BFP; Digital droplet PCR; Episomal plasmid; In vivo recombination; Interclonal variability; P. pastoris; Synthetic biology; panARS

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pichia / Plásmidos / Recombinación Genética / Proteínas Recombinantes / Vectores Genéticos Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Singapur

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