Deficiency of N-myristoylation reveals calcineurin activity as regulator of IFN-γ-producing γδ T cells.
J Leukoc Biol
; 101(4): 1005-1014, 2017 04.
Article
en En
| MEDLINE
| ID: mdl-28062573
ABSTRACT
γδ T cell subsets can be characterized, in part, by their secretion of select proinflammatory cytokines. The molecular mechanisms driving the diverse fates of γδ T cells have not been elucidated. We have previously shown that the attachment of myristic acid to the N-terminal glycine of proteins, termed N-myristoylation, is essential for αß T cell development and activation. Here, we explore the potential role of this lipid modification on the activation of γδ T cells. In the absence of N-myristoylation, the CD27+ γδ T cell subset was dominantly affected. The cells produced high levels of IFN-γ upon stimulation. In addition, they were more sensitive to inhibition of the CaN-Nfat pathway than were γδ T cells with myristoylated CaN. N-Myristoylation was found to modulate activity of phosphatase CaN, a regulator of Nfat. In summary, the CaN-Nfat pathway regulates development and function of IFN-γ-producing γδ T cells, and its balanced activity is strongly dependent on CaN N-myristoylation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Interferón gamma
/
Receptores de Antígenos de Linfocitos T gamma-delta
/
Ácido Mirístico
/
Calcineurina
Límite:
Animals
Idioma:
En
Revista:
J Leukoc Biol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania