Inflammatory Stroke Extracellular Vesicles Induce Macrophage Activation.
Stroke
; 48(8): 2292-2296, 2017 08.
Article
en En
| MEDLINE
| ID: mdl-28536169
ABSTRACT
BACKGROUND AND PURPOSE:
Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs.METHODS:
EVs were quantified and analyzed in the sera of patients after an acute stroke (<24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography mass-spectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression.RESULTS:
EV number, but not size, is significantly increased in stroke patients when compared to age-matched controls. Proteomic analysis reveals an overall increase in acute phase proteins, including C-reactive protein. EV fractions applied to monocyte-differentiated macrophage cultures induced inflammatory gene expression.CONCLUSIONS:
Together these data show that EVs from stroke patients are proinflammatory in nature and are capable of inducing inflammation in immune cells.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Mediadores de Inflamación
/
Accidente Cerebrovascular
/
Vesículas Extracelulares
/
Activación de Macrófagos
/
Macrófagos
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Aged
/
Aged80
/
Female
/
Humans
/
Male
País/Región como asunto:
Europa
Idioma:
En
Revista:
Stroke
Año:
2017
Tipo del documento:
Article