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Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA.
Kubo, Minoru; Nango, Eriko; Tono, Kensuke; Kimura, Tetsunari; Owada, Shigeki; Song, Changyong; Mafuné, Fumitaka; Miyajima, Ken; Takeda, Yoshihiro; Kohno, Jun Ya; Miyauchi, Naoya; Nakane, Takanori; Tanaka, Tomoyuki; Nomura, Takashi; Davidsson, Jan; Tanaka, Rie; Murata, Michio; Kameshima, Takashi; Hatsui, Takaki; Joti, Yasumasa; Neutze, Richard; Yabashi, Makina; Iwata, So.
Afiliación
  • Kubo M; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Nango E; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Tono K; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
  • Kimura T; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Owada S; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Song C; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Mafuné F; Department of Basic Science, School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan.
  • Miyajima K; Department of Basic Science, School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan.
  • Takeda Y; East Tokyo Laboratory, Genesis Research Institute, Inc., Futamata, Ichikawa, Chiba 272-0001, Japan.
  • Kohno JY; Department of Chemistry, School of Science, Gakushuin University, Mejiro, Toshima, Tokyo, Japan.
  • Miyauchi N; Research Center for Advanced Measurement and Characterization, National Institute for Materials Science, Sengen, Tsukuba, Ibaraki 305-0047, Japan.
  • Nakane T; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Tanaka T; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Nomura T; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Davidsson J; Department of Chemistry, Ångström Laboratory, Uppsala University, Uppsala, Sweden.
  • Tanaka R; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Murata M; JST-ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
  • Kameshima T; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
  • Hatsui T; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Joti Y; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
  • Neutze R; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, SE-40530 Gothenburg, Sweden.
  • Yabashi M; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
  • Iwata S; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
J Synchrotron Radiat ; 24(Pt 5): 1086-1091, 2017 Sep 01.
Article en En | MEDLINE | ID: mdl-28862633
X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: J Synchrotron Radiat Asunto de la revista: RADIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: J Synchrotron Radiat Asunto de la revista: RADIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Japón