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Circulating endothelial cell count: a reliable marker of endothelial damage in patients undergoing hematopoietic stem cell transplantation.
Almici, C; Skert, C; Bruno, B; Bianchetti, A; Verardi, R; Di Palma, A; Neva, A; Braga, S; Piccinelli, G; Piovani, G; Malagola, M; Bernardi, S; Giaccone, L; Brunello, L; Festuccia, M; Baeten, K; Russo, D; Marini, M.
Afiliación
  • Almici C; Department of Trasfusion Medicine, Laboratory for Stem Cells Manipulation and Cryopreservation, ASST Spedali Civili, Brescia, Italy.
  • Skert C; Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, University of Brescia, ASST Spedali Civili, Brescia, Italy.
  • Bruno B; BMT Unit, Department of Oncology, AOU Città della Salute e della Scienza di Torino, Torino, Italy.
  • Bianchetti A; Department of Trasfusion Medicine, Laboratory for Stem Cells Manipulation and Cryopreservation, ASST Spedali Civili, Brescia, Italy.
  • Verardi R; Department of Trasfusion Medicine, Laboratory for Stem Cells Manipulation and Cryopreservation, ASST Spedali Civili, Brescia, Italy.
  • Di Palma A; Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, University of Brescia, ASST Spedali Civili, Brescia, Italy.
  • Neva A; Department of Trasfusion Medicine, Laboratory for Stem Cells Manipulation and Cryopreservation, ASST Spedali Civili, Brescia, Italy.
  • Braga S; Department of Trasfusion Medicine, Laboratory for Stem Cells Manipulation and Cryopreservation, ASST Spedali Civili, Brescia, Italy.
  • Piccinelli G; Department of Transfusion Medicine, ASST Spedali Civili, Brescia, Italy.
  • Piovani G; Biology and Genetics Division, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
  • Malagola M; Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, University of Brescia, ASST Spedali Civili, Brescia, Italy.
  • Bernardi S; Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, University of Brescia, ASST Spedali Civili, Brescia, Italy.
  • Giaccone L; BMT Unit, Department of Oncology, AOU Città della Salute e della Scienza di Torino, Torino, Italy.
  • Brunello L; BMT Unit, Department of Oncology, AOU Città della Salute e della Scienza di Torino, Torino, Italy.
  • Festuccia M; BMT Unit, Department of Oncology, AOU Città della Salute e della Scienza di Torino, Torino, Italy.
  • Baeten K; Global Scientific and Medical Affairs, Janssen Diagnostics, Beerse, Belgium.
  • Russo D; Chair of Hematology, Unit of Blood Diseases and Stem Cell Transplantation, University of Brescia, ASST Spedali Civili, Brescia, Italy.
  • Marini M; Department of Transfusion Medicine, ASST Spedali Civili, Brescia, Italy.
Bone Marrow Transplant ; 52(12): 1637-1642, 2017 Dec.
Article en En | MEDLINE | ID: mdl-28892085
ABSTRACT
The physio-pathologic interrelationships between endothelium and GvHD have been better elucidated and have led to definition of the entity 'endothelial GvHD' as an essential early phase prior to the clinical presentation of acute GvHD. Using the CellSearch system, we analyzed circulating endothelial cells (CEC) in 90 allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients at the following time-points T1 (pre-conditioning), T2 (pre-transplant), T3 (engraftment), T4 (onset of GvHD) and T5 (1 week after steroid treatment). Although CEC changes in allo-HSCT represent a dynamic phenomenon influenced by many variables (that is, conditioning, immunosuppressive treatments, engraftment syndrome and infections), we showed that CEC peaks were constantly seen at onset of acute GvHD and invariably returned to pre-transplant values after treatment response. Since we showed that CEC changes during allo-HSCT has rapid kinetics that may be easily missed if blood samples are drawn at pre-fixed time-points, we rather suggest an 'on demand' evaluation of CEC counts right at onset of GvHD clinical symptoms to possibly help differentiate GvHD from other non-endothelial complications. We confirm that CEC changes are a suitable biomarker to monitor endothelial damage in patients undergoing allo-transplantation and hold the potential to become a useful tool to support GvHD diagnosis (ClinicalTrials.gov NCT02064972).
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Endotelio Vascular / Trasplante de Células Madre Hematopoyéticas / Células Endoteliales / Enfermedad Injerto contra Huésped Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Bone Marrow Transplant Asunto de la revista: TRANSPLANTE Año: 2017 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Endotelio Vascular / Trasplante de Células Madre Hematopoyéticas / Células Endoteliales / Enfermedad Injerto contra Huésped Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Bone Marrow Transplant Asunto de la revista: TRANSPLANTE Año: 2017 Tipo del documento: Article País de afiliación: Italia