Chromatographic method development and validation for the determination of valsartan in biological fluid.
Pak J Pharm Sci
; 30(6(Supplementary)): 2355-2362, 2017 Nov.
Article
en En
| MEDLINE
| ID: mdl-29188769
ABSTRACT
A swift, precise and simple HPLC bioanalytical technique with UV detection was established and validated for quantitative estimation of valsartan in human plasma. The analyte was separated from plasma by protein precipitation with acetonitrile and chromatographically separated on Zorbax SB-C18 (5µm, 4.6mm × 15cm) column. The solvent mixture system consisting of acetonitrile, water and glacial acetic acid (40591 v/v), was pumped using isocratic mode at 1mL/min flow rate. Samples' detection of drug was made spectrophotometrically at a wavelength of 264nm. The analyte response was instituted to be linear from 0.06 to 8µg/mL with a regression value of 0.999. The accuracy of the proposed method was ranged between 97.2-100.3% with 5% RSD. The analytical recovery (>95%) was consistently observed and satisfactory sample stability was also found at different environmental conditions. In conclusion the reported bio-analytical method is easy and robust that was successfully utilized in estimation of valsartan in a pharmacokinetic study.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrofotometría Ultravioleta
/
Cromatografía Líquida de Alta Presión
/
Bloqueadores del Receptor Tipo 1 de Angiotensina II
/
Valsartán
Límite:
Humans
/
Male
Idioma:
En
Revista:
Pak J Pharm Sci
Asunto de la revista:
FARMACIA
/
FARMACOLOGIA
/
QUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Pakistán