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Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2.
Peng, Yingying; Xie, Menghan; Duan, Xingxiang; Hu, Liang; Yu, Juan; Zeng, Sicong; Wang, Yang; Ouyang, Qi; Lu, Guangxiu; Lin, Ge; Sun, Yi.
Afiliación
  • Peng Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Xie M; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Duan X; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Hu L; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
  • Yu J; National Engineering and Research Center of Human Stem Cells, Changsha, China.
  • Zeng S; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China.
  • Wang Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Ouyang Q; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
  • Lu G; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
  • Lin G; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
  • Sun Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
Stem Cell Res ; 28: 172-176, 2018 04.
Article en En | MEDLINE | ID: mdl-29501955
ABSTRACT
The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides optimal prospects of application to in vivo stem cell tracing. Luciferin served as a substrate to detect the activity of luciferase, and luciferase expression was measured by quantitative PCR. Characterization assays suggested that the NERCe002-A-2 cell line expresses typical markers of pluripotency and can form the 3 germ layers in vivo.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Células Madre Embrionarias Humanas / Luciferasas Límite: Animals / Humans / Male Idioma: En Revista: Stem Cell Res Año: 2018 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Células Madre Embrionarias Humanas / Luciferasas Límite: Animals / Humans / Male Idioma: En Revista: Stem Cell Res Año: 2018 Tipo del documento: Article País de afiliación: China