Cellspecific and roastingdependent regulation of the Keap1/Nrf2 pathway by coffee extracts.
Mol Med Rep
; 17(6): 8325-8331, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29693701
ABSTRACT
Coffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted and the corresponding green one, were investigated for their effect on nuclear factor erythroid 2related factor 2 (Nrf2) target gene expression in myoblasts and endothelial cells using quantitative PCR. The tested concentrations were noncytotoxic and led to improved redox cell status, as was evident by increased reduced glutathione (GSH) levels. In both cell lines, the roasted extract upregulated gene expression more readily than its green counterpart leading to increased NAD(P)H quinone dehydrogenase 1 and γglutamyl cysteine ligase catalytic subunit, among others. The green extract had a mixed effect on the endothelial cells, while, as regards the myoblasts it caused the downregulation of some Nrftarget genes. Therefore, a potential dose and roastingdependent mechanism is proposed in the current study, accounting for coffee's antioxidant activity.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Transducción de Señal
/
Café
/
Factor 2 Relacionado con NF-E2
/
Proteína 1 Asociada A ECH Tipo Kelch
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article
País de afiliación:
Grecia