Identification of acetylated derivatives of zearalenone as novel plant metabolites by high-resolution mass spectrometry.
Anal Bioanal Chem
; 410(22): 5583-5592, 2018 Sep.
Article
en En
| MEDLINE
| ID: mdl-29707753
ABSTRACT
Zearalenone (ZEN) major biotransformation pathways described so far are based on glycosylation and sulfation, although acetylation of trichothecenes has been reported as well. We investigated herein the ZEN acetylation metabolism route in micropropagated durum wheat leaf, artificially contaminated with ZEN. We report the first experimental evidence of the formation of novel ZEN acetylated forms in wheat, attached both to the aglycone backbone as well as on the glucose moiety. Thanks to the advantages provided by high-resolution mass spectrometry, identification and structure annotation of 20 metabolites was achieved. In addition, a preliminary assessment of the toxicity of the annotated metabolites was performed in silico focusing on the toxicodynamic of ZEN group toxicity. All the metabolites showed a worse fitting within the estrogen receptor pocket in comparison with ZEN. Nevertheless, possible hydrolysis to the respective parent compounds (i.e., ZEN) may raise concern from the health perspective because these are well-known xenoestrogens. These results further enrich the biotransformation profile of ZEN, providing a helpful reference for assessing the risks to animals and humans. Graphical abstract á
.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Triticum
/
Zearalenona
/
Espectrometría de Masas en Tándem
/
Fusarium
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia